Advantages: Overcoming the deficiencies of the E. coli expression system, capable of rapid reproduction, high-density fermentation, and post-translational protein modification and processing.
Disadvantages: the lack of a strong and strictly regulated promoter, the secretion efficiency is low, especially for target protein molecules with a molecular mass greater than 30KD are hardly secreted. Not suitable for high density cultivation.
Methanol yeast expression system: It has the function of post-translational processing, and has an intracellular environment and sugar chain processing system suitable for the correct folding of eukaryotic gene products. It can also secrete the target gene product into the culture medium, which is conducive to purification and so on.
The yeast expression system is a powerful tool for studying the expression and analysis of eukaryotic proteins. It has post-transcriptional processing and modification functions and is suitable for the stable expression of functional foreign proteins. Compared with insect expression systems and mammalian expression systems, yeast expression systems are simple to operate, low in cost, and can be fermented on a large scale. They are ideal tools for the production of recombinant eukaryotic proteins.
Saccharomyces cerevisiae expression system (problems of Saccharomyces cerevisiae system promoter, Saccharomyces cerevisiae secretion system, Saccharomyces cerevisiae glycosylation system, Saccharomyces cerevisiae expression system)
Methanol yeast expression system (advantages and disadvantages)
High expression level (the highest level system)
Post-translational modification of products: glycosylation, phosphorylation, acyl lipidation
The degree of over-glycosylation is less than that of Saccharomyces cerevisiae (8-15 vs 100-150 mannose)
The product can be folded correctly and secreted efficiently (the highest secretion expression system)
Simple inorganic salt medium can be used for high-density fermentation and large biomass.
Simple laboratory and industrial operation
Can not meet the strict glycosylation of structural requirements
Other yeast expression systems
Medicilon researchers have established a mature yeast expression and purification service platform to provide high-quality recombinant protein expression and purification services in Pichia and Saccharomyces cerevisiae.
Yeast Expression System Service Items:
Construction of recombinant yeast expression plasmid;
Electric transformation of recombinant plasmid;
Recombinant expression screen;
Small-scale protein expression and purification (2L medium culture product);
Large-scale protein expression and purification (above 10L).
contact us
Email: marketing@Medicilon.com
Phone: 86-021-58591500
Website: www.medicilon.com
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