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Address: 20 Maguire Road, Suite 103, Lexington, MA 02421(America)
Tel: +1(781)535-1428
Address: Allia Future Business Centre Kings Hedges Road Cambridge CB4 2HY, UK
Tel: 0044 7790 816 954
Email: marketing@medicilon.com
Address: No.585 Chuanda Road, Pudong New Area, Shanghai (Headquarters)
Postcode: 201299
Tel: +86 (21) 5859-1500 (main line)
Fax: +86 (21) 5859-6369
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Global：
Email:marketing@medicilon.com
+1(781)535-1428(U.S.)
0044 7790 816 954 (Europe)
China：
Email: marketing@medicilon.com.cn
Tel: +86 (21) 5859-1500
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Services we offer
Items and Steps| Step | Content | Duration | Remark |
|---|---|---|---|
| 1 | Construction of recombinant yeast expression plasmid | 2 weeks | Different carriers available; different affinity tags; secretion or intracellular expression |
| 2 | Electrotransformation of recombinant plasmid | 1 week | Different expression bacterial strains available; 5-10 clones for PCR identification |
| 3 | Screening of recombinant expressors | 3-4 weeks | 5-10 clones for screening, recombinants screening based on Zeocin antibiotic concentration, and identification of recombinant protein expressors by Western Blot assays |
| 4 | Expression and purification of small-scale proteins | 2-4 weeks | Optimize culture medium, cell density, methanol concentration, induction time, etc. |
| 5 | Expression and purification of large-scale proteins | Determined based on the expressed volume | Shake flask or culture fermentor available |
You can choose any of the above experiments, or a combination of related experiments, according to your requirements.
Affinity purification, ion column purification, hydrophobic packing purification, or molecular sieve purification are available as purification options.
The nature of proteins, as well as the expression system selected, will affect the purity and activity of the final products.
Relevant laboratories
Relevant articles
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