Over 20 years of experience in R&D

01 Drug Discovery

Chemistry Research

Biology Research

Early Pharmacokinetics

02 Pharmaceutical Research

Intermediates

Active Pharmaceutical Ingredients

Pharmaceutical preparation

Analytical Method Development and Quality Control

CMC Filings

Analytical Testing Center

03 Preclinical Research

Pharmacology & Pharmacodynamics

Drug Safety Evaluation

DMPK

Bioanalysis

IND Filings

01 One-stop Integrated Services

Liposome Drug Development

Drug Discovery

Pharmaceutical Research

Preclinical Research

Botanical Drug Preclinical R&D

02 Innovative Drug Services

ADC

PROTAC

mRNA Vaccine

Cellular Immunotherapies

Nucleic Acid Drug

Peptide Drug Conjugate (PDC)

Antibody

03 Key Technical Services for Drug R&D

Cytokine and Biomarker Detection

Metabolite Identification (MetID)

Drug Forming Research

Flow Cytometry Technology

SEND Format Conversion

Flow Sorting

NanoString nCounter Detection

Routes of Drug Administration

04 Drug Efficacy Evaluation for Common Diseases

IO Pharmacodynamic Model

Inflammatory and Immune Diseases

Rodent Cerebrovascular Disease

05 Advanced Preparation R&D

Inhalation drug

Ophthalmic drug

Skin Topical Preparation

06 Targeted Drugs R&D Services

STAT3-targeted Drugs R&D Service

GLP-1 New Drug R&D Service

KRAS-targeted Drugs R&D Service

2024-09-13Medicilon Events

Medicilon Non-tumor Pharmacology Service Platform

Medicilon boasts 260+ evaluation models for non-tumor diseases, covering the inflammatory & immune diseases, CNS diseases, digestive diseases, , Metabolic diseases, cardiovascular diseases and other diseases.

Learn more

2024-08-29Medicilon Events

Medicilon Expands Global Presence with Official Opening of Second R&D Center in Massachusetts

In August 2024, Medicilon USA Corp (Medicilon)officially commenced operations at its second R&D center in Massachusetts, USA.

Medicilon 688202

RMB

As of Beijing time The data is from a third-party organization and is only for reference.

For actual information, please refer to：www.eastmoney.com

CLIENT CENTER

Intellectual Property Protection

U.S.

Address: 20 Maguire Road, Suite 103, Lexington, MA 02421(America)

Tel: +1(781)535-1428

Address: Allia Future Business Centre Kings Hedges Road Cambridge CB4 2HY, UK

Tel: 0044 7790 816 954

Email: marketing@medicilon.com

China

Address: No.585 Chuanda Road, Pudong New Area, Shanghai (Headquarters)

Postcode: 201299

Tel: +86 (21) 5859-1500 (main line)

Fax: +86 (21) 5859-6369

Shanghai Public Network Security File No. 31011502018888 | Website Map

Drug Discovery

FAQs

HomeservicesDrug DiscoveryBiology ResearchStructural BiologyProtein Expression and PurificationCHO Cell Protein Expression System

CHO Cell Protein Expression System

Featuring protein folding and post-translational modification, mammalian protein expression systems can produce proteins that closely resemble natural proteins, ensuring identical biological activity and characteristics. Therefore, mammalian cell expression systems are extensively utilized in the R&D and production of recombinant protein drugs, particularly therapeutic recombinant monoclonal antibody drugs.

Download

Services we offer

Utilizing a serum-free culture system, our mammalian protein expression system provides various eukaryotic protein services, including gene expression optimization, transient expression, stable expression, and antibody production.

Our researchers have established a robust mammalian protein expression service platform to facilitate the expression and purification of mammalian proteins.

Mammalian protein expression system services
Construction and preparation of recombinant mammalian cell expression vectorScreening of transient expression of recombinant proteins or antibodies in CHO and HEK293 cellsConstruction of recombinant protein or antibody-stabilized cell lines (using CHO-S and CHO-DG44 cell platforms)Culturing CHO suspension cells in bioreactors (3-30L)Culture services for hybridoma cells using roller bottle or bioreactor methods.

Items and Steps

Step	Content	Duration	Remark
1	Construction and preparation of recombinant mammalian cell expression vector	3 weeks	Use of different tags: Fc, Human Serum Albumin, 3xFlag, GST, 6His, etc.
2	Transient expression test of proteins or antibodies in CHO and HEK293 cells	2 weeks	Western Blot assay
3	Transient expression of recombinant proteins or antibodies in large volumes	4 week	PEI transient transfection
4	Construction of recombinant protein or antibody-stabilized cell lines	TBD	CHO-S and CHO-DG44 cell systems
5	Culture of CHO suspension cell bioreactor	TBD	3-30L

Rabbit IgG expressed in transient transfected 293 cells

Remarks

The conditioned medium was run on a 10% SDS-PAGE gel to separate proteins by mass and stained with Coomassie Blue dye.

Relevant laboratories

Relevant articles

Preparation of recombinant kinase E.Coli protein expression system Yeast Protein Expression System Insect Cell Protein Expression System CHO Cell Protein Expression System