Enzymology & Molecular Interactions

Services

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  Coagulation Pathway Studies In Vitro
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Service we offer

• In Vitro Enzymology and Molecular Interactions Technology Platform
  Fluorescence analysis methods: LanthaScreen; AlphaScreen; Z’-LYTE; HTRF, TR-FRET, FP , etc.Luminescence analysis method: Kinase-Glo, ADP-Glo, among others;Absorption analysis method: UV and VI;Non-mark selection method: SPR (Biacore).
  
• Validated biochemical assays
  
  Enzymatic/Molecular Interaction Screening Platform

  PDE Family	Protease	Others
  PDE1A	Thrombin	AR
  PDE1B	FVIIa	Bcl-xl
  PDE2A	FIXa	BRD4
  PDE3A	Fxa	Cathepsin
  PDE4A1	FXIa	Cox1
  PDE4B2	FXIIa	Cox2
  PDE4C1	Trypsin	ER
  PDE4D3	Plasmin	FTO
  PDE5A1	TPA	HDAC1
  PDE6C	Urokinase	HDAC2
  PDE7A	Kallikern	HDAC3
  PDE8A	Chymotrypsin	HDAC4
  PDE10A1	TAFIa	HDAC5
  		HDAC6
  		HDAC8
  		HIF-1a
  		HIF-2a
  		HSP90
  		Kras-WT
  		Kras-G12C
  		Kras-G12D
  		PARP1
  		PARP2
  		PHD1
  		PHD2
  		PHD3
  		PPARα
  		PPARγ
  		PPARδ
  		ROR
  		ROS
  		RYR
  		SHP2
  		Tubulin
  		polymerization
  		Tyrosinase
  		WRN
  		XO
  		H+K+ATPase

Molecular Interactions Platform

• SPR
  Surface plasmon resonance (SPR) reveals interactions between proteins and other biomolecules through physical optical effects, helping clients understand molecular functions and screen effective molecules. SPR tests can replace and are superior to tests such as WB, ELISA, Co-IP, Pull-down, ChIP, EMSA, FRET, and yeast two-hybrid tests.
• Scope of application
  Micromolecule drug screeningOrganism function evaluationMacromolecule force detectionOther tests
• Detection indicators
  Binding specificity analysisKinetic analysisConcentration analysisAffinity analysis
• Biacore 8K
  Integration: Offers integrated solutions from selection to characterization.High throughput: Capable of selecting at least 2,300 samples in a day, and completing kinetic characterization of 64 pairs in 5 hours.High sensitivity: Provides high-quality data even for micromolecules or complex targets, accurately characterizing the dissociation rate of ultra-high affinity molecules.
  PD-1EPHA2IGF-1RPD-L1EPHA3HSAVEGFEPHA4FcRNNectin-1EPHA5FcRINectin-24-1BBFcRIINectin-3SHP2FcRIIINectin-4ATIIIFactor BNECL1EGFRHER2NECL2Gp130TfR1NECL3OX40EPCRNECL4TIGITSTAT1NECL5FOLR1STAT3EPHA1INSRSTAT5
             
  Figure 1. Nivolumab binding with PD-1
  Figure 2. Novoseven binding with TF
  Figure 3. EFNA1 binding with EphA2
  Figure 4. Novoseven binding with ATIII

  Validated targets：PD-1, PD-L1, VEGF, Nectin1, Nectin2, Nectin3, Nectin4, NECL1, NECL2, NECL3, NECL4, NECL5, EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, INSR, IGF-1R, HSA, FcRN, FcRI, FcRII, FcRIII, C1q, Factor B, HER2, Transferrin, EPCR, STAT3, STAT5, STAT1, 4-1BB, SHP2, ATIII, EGFR, gp130

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