Enzymology & Molecular Interactions

Medicilon's Biology Department has rich experience in the field of in vitro biology, offering a comprehensive range of biological services including enzyme-level measurements, cell-level measurements, cell biology, biochemistry, in vitro isotope measurements, establishment of stable cell lines, gene knockout, RNAi, and MicroRNA technology.

Services

Coagulation Pathway Studies In Vitro

Learn more

01

Service we offer

In Vitro Enzymology and Molecular Interactions Technology Platform
Fluorescence analysis methods: LanthaScreen; AlphaScreen; Z’-LYTE; HTRF, TR-FRET, FP , etc.Luminescence analysis method: Kinase-Glo, ADP-Glo, among others;Absorption analysis method: UV and VI;Non-mark selection method: SPR (Biacore).

Validated biochemical assays

Enzymatic/Molecular Interaction Screening Platform

PDE Family	Protease	Others
PDE1A	Thrombin	AR
PDE1B	FVIIa	Bcl-xl
PDE2A	FIXa	BRD4
PDE3A	Fxa	Cathepsin
PDE4A1	FXIa	Cox1
PDE4B2	FXIIa	Cox2
PDE4C1	Trypsin	ER
PDE4D3	Plasmin	FTO
PDE5A1	TPA	HDAC1
PDE6C	Urokinase	HDAC2
PDE7A	Kallikern	HDAC3
PDE8A	Chymotrypsin	HDAC4
PDE10A1	TAFIa	HDAC5
		HDAC6
		HDAC8
		HIF-1a
		HIF-2a
		HSP90
		Kras-WT
		Kras-G12C
		Kras-G12D
		PARP1
		PARP2
		PHD1
		PHD2
		PHD3
		PPARα
		PPARγ
		PPARδ
		ROR
		ROS
		RYR
		SHP2
		Tubulin
		polymerization
		Tyrosinase
		WRN
		XO
		H+K+ATPase

Molecular Interactions Platform

As a high-throughput testing system, Biacore 8K can assist clients in analyzing a large number of samples quickly. Medicilon’s Biology Department is equipped with Biacore 8K and an experienced team eager to support clients with their SPR tests.

SPR
Surface plasmon resonance (SPR) reveals interactions between proteins and other biomolecules through physical optical effects, helping clients understand molecular functions and screen effective molecules. SPR tests can replace and are superior to tests such as WB, ELISA, Co-IP, Pull-down, ChIP, EMSA, FRET, and yeast two-hybrid tests.
Scope of application
Micromolecule drug screeningOrganism function evaluationMacromolecule force detectionOther tests
Detection indicators
Binding specificity analysisKinetic analysisConcentration analysisAffinity analysis
Biacore 8K
Integration: Offers integrated solutions from selection to characterization.High throughput: Capable of selecting at least 2,300 samples in a day, and completing kinetic characterization of 64 pairs in 5 hours.High sensitivity: Provides high-quality data even for micromolecules or complex targets, accurately characterizing the dissociation rate of ultra-high affinity molecules.
PD-1EPHA2IGF-1RPD-L1EPHA3HSAVEGFEPHA4FcRNNectin-1EPHA5FcRINectin-24-1BBFcRIINectin-3SHP2FcRIIINectin-4ATIIIFactor BNECL1EGFRHER2NECL2Gp130TfR1NECL3OX40EPCRNECL4TIGITSTAT1NECL5FOLR1STAT3EPHA1INSRSTAT5

Figure 1. Nivolumab binding with PD-1
Figure 2. Novoseven binding with TF
Figure 3. EFNA1 binding with EphA2
Figure 4. Novoseven binding with ATIII
Validated targets：PD-1, PD-L1, VEGF, Nectin1, Nectin2, Nectin3, Nectin4, NECL1, NECL2, NECL3, NECL4, NECL5, EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, INSR, IGF-1R, HSA, FcRN, FcRI, FcRII, FcRIII, C1q, Factor B, HER2, Transferrin, EPCR, STAT3, STAT5, STAT1, 4-1BB, SHP2, ATIII, EGFR, gp130

Relevant laboratories

Relevant articles

Coagulation Pathway Studies In Vitro