E.Coli protein expression system

Prokaryotic expression system (E. coli expression system) is a classical system developed earlier and widely used in gene expression technology. In recent decades, the E. coli expression system has been continuously developed and improved, and has been widely adopted by scientific research and industrial users for expressing various recombinant proteins. Compared with other expression systems, it is characterized by high expression levels of target genes, short culture periods, strong pollution resistance, and low cost.

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Services we offer

Our researchers have established a mature E.coli expression service platform to provide the expression and purification of various recombinant proteins and their complexes in Escherichia coli.

Prokaryotic protein expression system (for E.coli) services:
Construction of recombinant plasmids;Recombinant expression testing;Soluble expression testing;Expression and purification of small-scale proteins (cultured product in medium 2L);Expression and purification of large-scale proteins (over 10L)

Items and Steps

Step	Content	Duration	Remark
1	Construction of recombinant plasmid	2 weeks	Different carriers available; different tags include His, Trx, GST, SUMO, etc.
2	Recombinant expressor testing	1 week	Different expression bacterial strains available
3	Soluble expression testing	1-2 weeks	Different inducers and induction temperatures available, or a mutant construction based on point mutation of protein structure or deletion mutation
4	Expression and purification of small-scale proteins	1-2 weeks	In case of inclusion body expression, the time and methodology will be adjusted.
5	Expression and purification of large-scale proteins	Determined based on the expressed volume	Shake flask or culture fermentor available

Remarks

You can choose any of the above experiments, or the combination of related experiments, according to your requirements.

Affinity purification, ion column purification, hydrophobic packing purification, or molecular sieve purification are available for the purification options.

The nature of proteins, as well as the expression system selected, will affect the purity and activity of the final products.

Relevant laboratories

Relevant articles

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