Cell Biology

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  In Vitro Evaluation of PROTAC Molecules
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Services we offer

• Cell Assay Analysis
  Cytotoxic assay-CTG,CCK8,MTTOris™ Cell Migration AssayTranswell cell migration assayCell necrosis assay - TNF+SM164+z-VADApoptosis assay-FACSCell cycle assay-FACSPhosphorylated protein assay-Western blot/MSD/HTRFConfocal-based ADC endocytosis assaypHrodo-based ADC endocytosis assayADC bystander effect-FACSNanoBiT/NanoBRET reporter gene assayDual luciferase reporter gene assayCytokine release assay-ELISA/Luminex/MSDHuman Blood Protein Phosphorylation Assay-FACSProtein degradation assay-HiBiT/In cell Western/WB/HTRFTricomplex formation-NanoBRETpsiCHECK2 reporter gene assay-siRNA screeningLiver tumor cell transfection/uptake assay-siRNA screeningPrimary hepatocyte transfection/uptake assay-siRNA screeningHepatocyte toxicity assay-siRNA off-target assayRNA-seq-siRNA off-target assayCar-T cell killing assay-FACSCar-T cell cytokine release assayCell binding assay-antibody screening-FACSADCC/ADCP/CDC-Antibody Screen-FACSSecond messenger assay - cAMP/cGMP/IP3/Ca2+
• Cell Level Analysis Services From Different Sources
  African green monkeyHumanHomo sapiensHamsterMouseRabbitRatSyrian golden

  African green monkey

  Cells	Cell line description	Source	Tissue
  Vero	Normal kidney epithelial cells	African green monkey	kidney
  Vero E6		African green monkey	kidney

  Human

  Cells	Cell line description	Source	Tissue
  22RV1	Carcinoma	Human	Prostate
  293T		Human	Kidney
  786-O	Renal cell carcinoma	Human	Kidney
  A  375	Melanoma	Human	Skin
  A 431	Epidermoid carcinoma	Human	Skin
  A 498	Renal cell carcinoma	Human	Kidney
  A 549	Adenocarcinomic alveolar basal epithelial cells	Human	Lung
  A2780	Carcinoma	Human	Ovary
  A-427	Carcinoma	Human	Lung
  A-673	Ewing's Sarcoma	Human	Muscle
  AGS	Gastric adenocarcinoma	Human	Stomach
  AN3CA	Human endometrial adenocarcinoma	Human	uterus
  AsPC-1	Adenocarcinoma  pancreas	Human	Pancreas
  BEL-7402	Hepatoma cells	Human	Liver
  BT474	Breast carcinoma	Human	Breast
  BT-549	Ductal carcinoma	Human	Breast
  BxPC-3	Pancreatic cancer cells	Human	Pancreas
  C-33A	Adenocarcinoma	Human	Cervix
  Ca Ski	Adenocarcinoma	Human	Cervix
  Caco-2	Colorectal adenocarcinoma	Human	Colon
  Caki-1	Renal cell carcinoma	Human	Kidney
  Calu-3	Non-small-cell lung cancer	Human	Lung
  Capan-1	Adenocarcinoma	Human	Pancreas
  Capan-2	Pancreatic cancer cells	Human	Pancreas
  CCRF-CEM	T lymphoblastoid line	Human	Blood
  CFPAC-1	Pancreatic adenocarcinoma	Human	Pancreas
  CHP-212	Neuroblastoma	Human	Brain
  Colo 320	Colorectal adenocarcinoma	Human	Colon
  Colo 320DM	Colorectal adenocarcinoma	Human	Colon
  Colo205	Colorectal adenocarcinoma	Human	Colon
  Colo-829	Malignant melanoma	Human	Skin
  Daoy	medulloblastoma cells	Human	blood
  Daudi	Burkitt's    lymphoma	Human	Blood
  DLD-1	Colorectal adenocarcinoma	Human	Colon
  DMS 114	Carcinoma	Human	lung
  DOHH-2	diffuse_large_B_cell_lymphoma	Human	haematopoietic_and_lymphoid_tissue
  DU145	Prostate carcinoma	Human	Prostate
  EJ	Bladder carcinoma	Human	Bladder
  FaDu	squamous cell carcinoma	Human	Pharynx
  H-22	Hepatocarcinoma	Human	Liver
  HCA-7	Carcinoma	Human	Colon
  HCC-1500	Carcinoma	Human	Breast
  HCC-1569	primary metaplastic carcinoma	Human	Breast
  HCC1937	Primary ductal carcinoma	Human	Breast
  HCC827	Non-small cell lung cancer	Human	Lung
  HCT116	Colorectal adenocarcinoma	Human	Colon
  HCT-15	Colorectal adenocarcinoma	Human	Colon
  HCT-8	Human colorectal cancer cellline	Human	Colon
  HCvEpC	Normal Cervical Epithelial Cell	Human	Cervix
  HEK293		Human	Kidney
  HEL	Leukemic cell	Human	Peripheral blood
  Hela	Adenocarcinoma	Human	Cervix
  Hep G2	Hepatocellular carcinoma	Human	Liver
  Hep3B (Hep3B2.1-7)	Hepatoma cells	Human	Liver
  HFL1	Fetal lung fibroblast	Human	Lung
  HL-60	Acute promyeloblastic leukemia	Human	Blood
  HPAF-II	Adenocarcinoma	Human	Pancreas
  HS 746T	Gastric carcinoma	Human	Stomach
  HS-578-T	Ductal Breast carcinoma	Human	Breast
  HT-1080	Fibrosarcoma	Human	Connective tissue
  HT-29	Colorectal adenocarcinoma	Human	Colon
  HUH 7	hepatocarcinoma	human	liver
  HUVEC	human umbilical vein endothelial cell	Human	Umbilical Vascular Endothelium
  Jeko-1	Mantle Cell Lymphoma	Human	Blood
  Jurkat clone E6-1	Acute T cell leukemia	Human	Blood
  K-562	Chronic myelogenous leukemia (CML)	Human	Blood
  Karpas299	T cell non-Hodgkin Ki-positive large cell lymphoma (CD30+ anaplastic large cell lymphoma	Human	Blood
  Kasumi-1	Human acute myelogenous leukemia cells	Human	Blood
  KATOIII	human  gastric cancer cell	Human	Stomach
  KB	Epithelial Carcinoma	Human	Mouth
  KM12-luc	Carcinoma	Human	Colon
  KMS-11		Human	Bone; Marrow
  KYSE150	squamous	Human	esophagus
  KYSE-520	Human esophageal squamous cell carcinoma	Human	esophageal
  L-02	Normal	human	Liver
  LIM-1215	Carcinoma	Human	Colon
  LNCap clone FGC(LNCap)	Carcinoma	Human	Prostate
  LS 174T	Colorectal adenocarcinoma	Human	Colon
  LX-2	Hepatic stellate cellS	Human	Liver
  M14	Melanoma	Human	Skin
  MCF-10A	Breast carcinoma	Human	Breast
  MCF-7	Breast adenocarcinoma	Human	Breast
  MDA-kb2	Stably Expresses an Androgen- and  Glucocorticoid-Responsive Reporter	Human	Breast
  MDA-MB-134VI		Human	Breast
  MDA-MB-157	Medulallary carcinoma	Human	Medulla
  MDA-MB-231	Breast adenocarcinoma	Human	Breast
  MDA-MB-435S	Breast carcinoma	Human	Breast
  MDA-MB-436	Breast carcinoma	Human	Breast
  MDA-MB-453	Breast carcinoma	Human	Breast
  MDA-MB-468	Breast adenocarcinoma	Human	Breast
  Mewo	human  malignant melanoma	Human	skin
  MG-63	Osteosarcoma	Human	Bone
  MHCC97L	Carcinoma	Human	Liver
  MiaPaCa-2	Ductal adenocarcinoma	Human	Pancreas
  Mino	Mantle cell lymphoma	Human	Blood
  MKN-45	Gastric carcinoma	Human	Stomach
  MM.1R	B lymphoblast	Human	Blood
  MM.1S	Immunoglobulin A lambda myeloma	Human	Blood
  Molm-13	Acute myeloid leukemia	Human	Blood
  MOLT-3	Acute T cell leukemia	Human	Blood
  MRC-5		Human	Lung
  MSTO-211H	Biphasic mesothelioma	Human	Lung
  MV-4-11	Biphenotypic B myelomonocytic leukemia	Human	Peripheral blood
  MX-1	Carcinoma	Human	Breast
  NCI-H1299	Non-small cell lung cancer	Human	Lung
  NCI-H2122		Human	Lung
  NCI-H441		Human	Lung
  NCI-H1373	stage 3A, adenocarcinoma	Human	lung
  NCI-H1650	Bronchoalveolar carcinoma	Human	Lung
  NCI-H1666	Bronchoalveolar carcinoma	Human	Lung
  NCI-H1703	squamous cell	Human	Lung
  NCI-H1838	Human non-small cell lung cancer cells	Human	lung
  NCI-H187	Small cell lung carcinoma	Human	Lung
  NCI-H1975	Non-small cell lung cancer	Human	Lung
  NCI-H2009	Stage 4, adenocarcinoma	Human	Lung
  NCI-H2030	adenocarcinoma; non-small cell lung cancer	Human	lung
  NCI-H211	Small cell lung carcinoma	Human	Lung
  NCI-H2170	squamous cell carcinoma	Human	Lung
  NCI-H2228	Non-small cell lung cancer	Human	Lung
  NCI-H226	Squamous cell carcinoma	Human	Lung
  NCI-H23	adenocarcinoma;non-small cell lung cancer	Human	lung
  NCI-H292	Mucoepidermoid pulmonary carcinoma	Human	Lung
  NCI-H3122	Non-small cell lung adenocarcinoma	Human	Lung
  NCI-H358	Non-small cell lung cancer	Human	Lung
  NCI-H460	Large cell lung cancer	Human	Lung
  NCI-H508	Human colorectal adenocarcinoma	Human	colon
  NCI-H520	Squamous cell carcinoma	Human	Lung
  NCI-H522	Non-small cell lung cancer	Human	Lung
  NCI-H69	Small cell lung cancer	Human	Lung
  NCI-H716	Colorectal adenocarcinoma	human	Colon
  NCI-H929	Plasmacytoma; myeloma	Human	Bone    Marrow
  NCI-N87	Gastric carcinoma	Human	Stomach
  OCI-LY10	B lymphoma cells	Human	Blood
  OPM-2	Multiple myeloma	Human	Peripheral blood
  OS-RC-2	renal cell carcinoma	Human	Kidney
  OVCAR-3	Ovarian carcinoma cell	Human	Ovary
  OVCAR-8	Adenocarcinoma	Human	Ovary
  PANC-1	Pancreatic carcinoma	Human	Pancreas
  PC-3	Prostate	Human	Prostate
  PC-9	Adenocarcinoma	Human	Lung
  PLC/PRF/5	Alexander cells	Human	liver
  PLC-PRF-5	Hepatoma	Human	Liver
  QGY-7703	Hepatocellular carcinoma	Human	Liver
  Raji	Burkitt's lymphoma	Human	Lymphoblast
  Ramos	Burkitt's lymphoma (American)	Human	B lymphocyte
  RKO	Colorectal adenocarcinoma	Human	Colon
  RPMI 8226	Myeloma cells	Human	Blood
  RT112/84		Human	Bladder
  Saos-2	Osteosarcoma cells	Human	Bone
  SCC-4	Squamous cell carcinoma	Human	Tongue
  SF268	Glioma	Human	Brain
  SGC7901	Gastric cancer	Human	Stomach
  SH-SY5Y	Neuroblastoma cells	Human	Neuron
  SiHa	Carcinoma	Human	Cervix
  SJSA-1	Osteosarcoma cells	Human	Bone
  SK-BR-3	Breast carcinoma	Human	Breast
  SK-HEP-1	Ascites adenocarcinoma	Human	Liver
  SK-MEL-28	Melanoma	Human	Skin
  SK-MEL-3	Melanoma	Human	Skin
  SK-MEL-31	Melanoma	Human	Skin
  SK-OV-3	Ovary, adenocarcinoma	Human	Ovary
  SK-OV-3 Red-Fluc	Ovary, adenocarcinoma	Human	Ovary
  SMC-1	Malignant Mesothelioma cells	Human	Pleural membrane
  SMMC-7721	Hepatoma cells	Human	Liver
  SNU16	Human gastric cancer cell	Human	Stomach
  SNU216	Gastric cancer	Human	Stomach
  SNU-387	Carcinoma	Human	liver
  SU.86.86	Pancreas Adenocarcinoma	Human	Pancreas
  SU-DHL-4	B lymphocyte	Human	Peritoneal effusion
  SW 1573	alveolar cell carcinoma	Human	lung
  SW1116	Dukes' type A, grade III, colorectal adenocarcinoma	Human	Colon
  SW480	human colorectal adenocarcinoma	Human	Colon
  SW620	Colorectal adenocarcinoma	Human	Colon
  T24	Carcinoma	Human	Urinary bladder
  THP-1	Acute monocytic leukemia	Human	Blood
  TT	medullary carcinoma	Human	thyroid
  U-2 OS	Osteosarcoma cells	Human	Bone
  U251	Glioma	Human	Brain
  U266B1	Myeloma; plasmacytoma	Human	Blood
  U87 MG-Red-Fluc cell line BW124577V	Glioblastoma; astrocytoma	Human	Brain
  U-87MG	Glioblastoma; astrocytoma	Human	Brain
  U-87MG luc	Glioblastoma; astrocytoma	Human	Brain
  U937	Macrophage cells	Human	Lymphoma
  WSU-DLCL2	Diffuse Large B Cell Lymphoma	Human	Blood
  ZR-75-1	Breast carcinoma	Human	Breast

  Homo sapiens

  Cells	Cell line description	Source	Tissue
  T47D	Ductal carcinoma	Homo  sapiens	Breast

  Hamster

  Cells	Cell line description	Source	Tissue
  CHL	Chinese hamster lung	Hamster	Lung
  UC10-4F10-11	B lymphocyte	Hamster	Blood

  Mouse

  Cells	Cell line description	Source	Tissue
  2PK-3	B lymphocyte	Mouse	Blood
  4T1	Animal stage IV human breast cancer.	Mouse	Breast
  4T1 luc	Animal stage IV human breast cancer.	Mouse	Breast
  A 20	B lymphoma cells	Mouse	Blood
  B16-F10	Melanoma	Mouse	Skin
  B16F10-luc2	Melanoma	Mouse	Skin
  Ba/F3	Mouse Pro B cells	mouse	Lymphocyte
  C127I	Mammary tumor RIII	Mouse	Breast
  C1498	Acute    myeloid leukemia	Mouse	Blood
  CMT-93	polyploid carcinoma	Mouse	Rectum
  CT26.WT	Carcinoma	Mouse	Colon
  E.G7-OVA	T lymphocyte	Mouse	Blood
  EL4	Lymphoma	Mouse	Blood
  EMT6	Carcinoma	Mouse	Breast
  Hepa1-6	Hepatocellular carcinoma	Mouse	Liver
  Hepa-1c1c7	Carcinoma	Mouse	Liver
  ID8	Ovarian cancer	Mouse	Ovary
  J558	B lymphocyte	Mouse	Blood
  JC	Adenocarcinoma	Mouse	Breast
  KLN 205	Squamous cell carcinoma	Mouse	Lung
  L1210	Lymphocytic leukemia	Mouse	Blood
  L5178-R (LY-R)	Lymphoma	Mouse	Lymphoblast
  L5178Y TK+/- clone	Lymphoma	Mouse	Lymphoblast
  L929	fibroblast	mouse	subcutaneous connective tissue
  LL/2 (LLC1)	Lewis carcinoma	Mouse	Lung
  LLC	Lewis carcinoma	Mouse	Lung
  MC-38	Colon carcinoma	Mouse	Colon
  MITC-STO	Embryo cell Type fibroblast	Mouse	Embryo
  MPC-11	Plasmacytoma cells	Mouse	Blood
  NIH/3T3	NIH Swiss mouse embryo	Mouse	Embryo
  P388D1	Vinorelbine-resistant leukemia	Mouse	Blood
  Raw264.7	Macrophage cells	Mouse	Ascites
  RENCA	Adenocarcinoma	Mouse	Kidney
  RM-1	Carcinoma	Mouse	Prostate
  S-180	Sarcoma	Mouse	Ascites
  Sp2/0;Sp2/0-Ag14	BALB/c  B lymphocyte, myeloma cells	Mouse	Blood
  WEHI-3	Leukemia	Mouse	Blood

  Rabbit

  Cells	Cell line description	Source	Tissue
  HIG 82	Periarticular soft tissue	Rabbit	Synovium

  Rat

  Cells	Cell line description	Source	Tissue
  HSC-T6	Hepatic stellate cells	Rat	Liver
  PC-12 (Low differentiation)	Pheochromocytoma	Rat	Adrenal    gland
  PC-12 (Highly differentiated)	Pheochromocytoma	Rat	Adrenal gland
  RASMC	fetal aortic smooth muscle cells	Rat	Aorta

  Syrian golden

  Cells	Cell line description	Source	Tissue
  BHK-21	Normal	Syrian golden	Kidney

• Radioisotope Analysis
  B-cell activation/B-cell proliferation assay-isotope method/fluorescence methodT-cell activation/T-cell proliferation assay-isotope method/fluorescence methodReceptor binding assay - isotope methodLigand binding assay - isotope methodFree uptake assay - isotope method

Feature Platform

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  Small Nucleic Acid Platform

  Contains：

  Sequence DesignIn vitro screeningOff-target effects

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  In Vitro Evaluation of PROTAC Molecules

  Contains：

  Formation analysis of binary and ternary complexesTarget ubiquitination analysisTarget degradation analysis

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  In vitro Pharmacodynamic Studies of Cellular Immunotherapies

  Contains：

  Efficacy testing of cell therapy (such as CAR-T):                    Tumor killing rate or proliferation inhibition rateIFN-γ expression levelPhenotype changes of CAR-T cells

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