X-ray-based Crystal Structural Biology

The measurement of the complete three-dimensional structure of a protein and its complex assembly is the scientific basis for studying the relationship between molecular structure and function in life activities, as well as for revealing the physical and chemical nature of life phenomena. X-ray diffraction of protein and its complex crystals is one of the main methods used to study the three-dimensional fine structure of biological macromolecules.

Combining with other disciplines, and especially driven by fields like structural genomics, protein crystallography extends from analyzing the simple three-dimensional structure of proteins to studying the structures of various biological macromolecules and complexes, focusing on the relationship between structure and function. In pharmaceutical R&D, protein crystallographic structure is widely and significantly used in structure-based novel drug design.

Services we offer

High-throughput-based selection of protein crystalsDiffraction data collection in protein synchrotron radiationProtein-compound co-crystallizationAnalysis of protein crystal structure

Items and Steps

Step	Content	Duration	Remark
1	High-throughput-based selection of protein crystals	4-8 weeks	Commercial selection kit, Mosquito selection
2	Diffraction data collection in protein synchrotron radiation	4-8 weeks	Diffraction data collection in synchrotron radiation for Shanghai
3	A. Protein-compound co-crystallization; B. Fragment selection	4-8 weeks	Compound-binding constant obtained via ITC and Biacore testing recommended
4	Analysis of protein crystal	Determined based on specific project	Phase analysis of molecular replacement and heavy metal replacement

Remarks

You can choose any of the above experiments, or a combination of related experiments, according to your requirements.

Case study-KRAS related assays

KRAS-G12D crystallization
Screening of KRAS^G12D
$Synchrotron X-ray diffraction data of the co-crystallization.webp$
Synchrotron X-ray diffraction data of KRAS^G12D
Compare the Structure of KRAS^G12D with 7RPZ, the green is PDB ID 7RPZ and the pink is the data from Medicilon. The data are very correlated.
KRAS-G12D-MRTX1133 co-crystallization
Screening of the co-crystallization
$Synchrotron X-ray diffraction data of KRASG12D.webp$
Synchrotron X-ray diffraction data of the co-crystallization
Compare the Structure of KRAS^G12D co-crystallization with MRTX1133(7RPZ, PDB), the green strucuture is PDB ID 7RPZ and the cyan is data from Medicilon. The data are very correlated.
Structure of KRAS^GI2D co-crystallized with MRTX1133 with GDP-bound