Insect Cell Protein Expression System

The baculovirus expression vector system (BEVS) has been widely used since 1983 for preparing various recombinant proteins, particularly recombinant kinases and protein complexes. Our researchers have developed a mature baculovirus-insect cell expression service that includes preparing recombinant baculoviruses, and expressing and purifying recombinant proteins and their complexes.

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Services we offer

Baculovirus vector expression system services:
Preparation of recombinant bacmid DNAPreparation of recombinant baculovirusDetermination of recombinant virus titerOptimization of recombinant protein expression conditionsExpression and purification of small-scale proteins (500ml culture)Expression and purification of large-scale proteins (optional)Insect cell culture in bioreactor (3-30L)

Items and Steps

Step	Content	Duration	Remark
1	Preparation of recombinant bacmid DNA	3 weeks	Use of different tags: GST, His, Flag, ...
2	Preparation of recombinant baculovirus	2 weeks
3	Determination of recombinant virus titer	2-3 days	Staining gp64 antibody methodology
4	Optimization of recombinant protein expression conditions	2 weeks	Effects of different MOI and PI on expression Select different cell lines (sf9, sf21, Hi5), according to requirements
5	Expression and purification of small-scale proteins (500ml-2L)	2-4 weeks	Provision of expressed cells is also acceptable
6	Expression and purification of large-scale proteins (over 10L)	Determine based on the expressed yield and volume	Shake flask or fermentor for culture available

Remarks：Any of the above experiments or a combination of related experiments can be selected according to your requirements.

Relevant laboratories

Relevant articles

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