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Address: 20 Maguire Road, Suite 103, Lexington, MA 02421(America)
Tel: +1(781)535-1428
Address: Allia Future Business Centre Kings Hedges Road Cambridge CB4 2HY, UK
Tel: 0044 7790 816 954
Email: marketing@medicilon.com
Address: No.585 Chuanda Road, Pudong New Area, Shanghai (Headquarters)
Postcode: 201299
Tel: +86 (21) 5859-1500 (main line)
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0044 7790 816 954 (Europe)
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Tel: +86 (21) 5859-1500
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Items and Steps| Step | Content | Duration | Remark |
|---|---|---|---|
| 1 | Preparation of recombinant bacmid DNA | 3 weeks | Use of different tags: GST, His, Flag, ... |
| 2 | Preparation of recombinant baculovirus | 2 weeks | |
| 3 | Determination of recombinant virus titer | 2-3 days | Staining gp64 antibody methodology |
| 4 | Optimization of recombinant protein expression conditions | 2 weeks | Effects of different MOI and PI on expression Select different cell lines (sf9, sf21, Hi5), according to requirements |
| 5 | Expression and purification of small-scale proteins (500ml-2L) | 2-4 weeks | Provision of expressed cells is also acceptable |
| 6 | Expression and purification of large-scale proteins (over 10L) | Determine based on the expressed yield and volume | Shake flask or fermentor for culture available |
Remarks：Any of the above experiments or a combination of related experiments can be selected according to your requirements.
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