Clinicopathological Study

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The Clinicopathology Laboratory

• Clinical Pathology is the study of disease in animals by examining specimens of blood, tissue and body fluids.
  Medicilon's Clinicopathology Laboratory is equipped with state-of-the-art analytical instruments including automatic biochemical analyzer, automatic hematology analyzer, automatic coagulation analyzer, automatic urine analyzer, and flow cytometer. The instruments in the laboratory feature stable performance, fast detection speed and reliable detection results.
  In addition, the laboratory is also available for various morphological testing projects, such as blood cell morphology testing, urine sediment cell morphology testing and marrow cell morphology testing.

Clinicopathology Laboratory Services

• Medicilon's Clinicopathology Laboratory can provide a range of services including biochemical analysis, hematology analysis, coagulation analysis, urine analysis, immunological analysis, urine sediment microscopy, cell morphology testing, and special biochemical analysis projects.
  Special biochemical analysis projects:
  High-sensitivity C-reactive protein (hs-CRP)Immunoglobulin A (IgA)Immunoglobulin G (IgG)Immunoglobulin M (IgM)Complement C3Complement C4
  Total hemoglobin (THb)Glycated hemoglobin (HbA1c)Glycated hemoglobin (HbA1c)
  Uric acid (UA)
  Alpha-amylase (AMY)Lipase (LPS)
  High-density lipoprotein cholesterol (HDL-C)Low-density lipoprotein cholesterol (LDL-C)
• The laboratory has participated in the room quality assessment by Shanghai Center for Clinical Laboratory and has been awarded certificates for years with all items qualified.
• 

  Hematology Analysis

  Blood cell analysis is an important testing item in hematology. To improve testing efficiency, the laboratory uses a blood analyzer pipeline for testing.The SYSMEX-XN-2000v automatic hematology analyzer features flow cytometry combined with laser technology and electrical impedance analysis methodology to confirm accurate and reliable results.

  Test items: CBC, DIFF and RET.It has 4 channels and 34 precision detection parameters.It features the PLT-F channel, L-WBC low-value white blood cell detection mode, which greatly improves the accuracy of sample testing results. The instrument can detect 27 species of animals (19 species, 8 subspecies), fully covering the species required for the experiment. In addition, customized analysis templates can be created according to specific animal species samples for specialized analysis. The fully automated blood analyzer can analyze nearly 100 samples per hour when operating in dual-mode, effectively saving time and labor costs. It supports both automatic and manual sample loading modes, and has whole blood and micro-blood modes. The use of micro-blood mode significantly reduces the amount of animal blood samples required for analysis.
• 

  Coagulation analysis

  Coagulation is the process of blood changing from a liquid state to a gel state. This process involves a series of enzymatic reactions and molecular polymerization processes with the participation of various coagulation factors. The automatic coagulation analyzer is mainly used to measure the plasma hemostasis and the detection of thrombus molecular markers of experimental animals. These indicators are closely related to various diseases of experimental animals, such as atherosclerosis, cardiovascular and cerebrovascular diseases, diabetes, arteriovenous thrombosis, etc.

  The Sysmex-CS-5100 automatic coagulation analyzer is a complete and excellent high-speed analysis system with reliable traceability.

  Test items: Prothrombin time, activated partial thromboplastin time, fibrinogen and thrombin time. It has four detection methods, multi-wavelength detection system reagent intelligent management, automatic barcode recognition and other functions.
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  Urinalysis

  Urinalysis is one of the three routine tests (blood, urine and stool). As an examination of excretions, the results can reflect the metabolic status of the body. It is an important indicator for the diagnosis of many diseases and is also a sign of kidney or urinary tract disease. Many early stage of kidney diseases can feature proteinuria or visible components in urine sediment.

  Test items: Color, appearance, pH, glucose, bilirubin, ketone body, specific gravity, protein, occult blood, urinary biliary origin, nitrite and white blood cells. The automatic quantitative point method is used to add samples to avoid contamination. Transmissive measurement is used to detect color and appearance, making the test results more accurate and reliable.

• Immunological analysis

  Immunological analysis is a method that utilizes the specific binding between antigens and antibodies in vitro, and then uses a flow cytometer to detect and analyze the desired substances. Analyzing lymphocyte subsets using flow cytometry is an important test in drug safety evaluation research. The flow cytometer in the Clinicopathology Laboratory is mainly used for lymphocyte phenotypic analysis of animal blood cells. By analyzing the quantity and function of various immune cell populations, it reflects changes in the body's immune system.

  

  BD FACSLyric flow cytometer is equipped with 3 lasers and 12 colors, which allows for a more efficient detection rate and more flexible color scheme. A more efficient and intelligent automatic sampling system that can perform continuous sampling and data collection with tube racks or 96-well plates, achieving up to 35,000 events/sec. More accurate light path self-adjustment. FACSLyric has a fluorescence compensation value update function once every 60 days. It also has a more precise optical self-adjustment and updates the fluorescence compensation values every 60 days. The advanced operating interface allows real-time monitoring of the testing status and indicators of each sample.

  Test items: T lymphocytes, B lymphocytes, NK cells, etc.
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  Biochemical assay

  Clinical biochemical indicators are mainly used to detect the metabolism of carbohydrates, lipids, proteins, and evaluate the urinary, hepatobiliary, cardiovascular, muscle and gastrointestinal systems. These detection indicators reflect the impact on the overall metabolism, nutrition and health status of the animal body in preclinical research of drugs.

  The fully automatic biochemical analyzer uses modular parallel work to quickly complete detection and report data; it has a more stable Na, K, CI electrode system and independent electrodes to make sample detection more accurate; the instrument has a built-in dry incubation system with constant temperature liquid circulation. Making temperature control more accurate and stable, and ensure the accuracy of detection data. 4000 photometric tests/hour, 900 electrolyte tests/hour (ion selective electrode method), 120 analysis projects can be performed simultaneously. With an additional independent retest track and a dual sampling system, it fully meets the requirements, making it more convenient, flexible, and safe.

  Test items: Liver and kidney function, myocardial function, blood sugar and blood lipid, electrolyte, etc.
• Morphological assay
  In terms of clinicopathology, microscopes are mainly used for：
  Examination of parasites in stool

  

  Observation of cell agglutination in in vitro hemolysis test

  

  Morphological cell classification of peripheral blood smear

  

  Morphological cell classification of marrow smear

  

  Morphological assays can provide reference for physical examination of parasites, confirm results  of partial in vitro hemolysis experiments, re-check of microscopic examination result of hematology analysis and proliferation degree and toxicity changes of marrow karyocytes of various animals.

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