Shanghai Medicilon Inc. can generate your over-expressing or knockdown stable cell line with the best quality and best price. We provide stable cell line generation service for research, biotechnology and pharmaceutical companies. Contact us via email : marketing@medicilon.com
Custom Stable Cell Line Generation Service Proceeds:
Phase I. Construct Generation (Optional)
Our professional scientists will generate constructs for your project. We will clone your target gene into lentiviral transfer vector. Lentivirus will be produced, concentrated and titrated after the constructs are confirmed by sequencing. Phase I takes about 3-4 weeks. However, we can also generate non-lentiviral vector constructs for your project if you need.
Phase II. Stable Cell Clones Selection
Perform transduction on target cells, select stable transduced cell clones. Phase II takes about 1-3 weeks depending on cell growth and antibiotics for selection.
Phase III. Gene Expression Evaluation
To evaluate the gene expression in stable transduced cell clones, our scientists will perform one or several assays including Western Blot, ELISA, real-time PCR or reporter assays, etc. Phase III usually takes about 2 weeks.
The services provided by AMSBIO for generation of cell lines include:
Integrating the desired gene or shRNA into the lentiviral vector
Generating the lentivirus particle with the desired gene
Transduce or infect the gene into the cell line needed
Selection of only high expression cells
Validate the integration of the gene by genomic PCR and validate the high-expression clone by Western Blot (if needed)
Preparation of two cryogenic preserved vials of stable cells along with documentation
Stable cell line is the best solution, not only to produce recombinant protein and antibody, but also for application in drug screenings and gene functional studies, etc. Depending on the type of gene expression and the gene construct, there are many different approaches for establishing stable cell lines.
Email : marketing@medicilon.com
Tel : +86 021 58591500
Tips: Above is part of stable cell line generation service and generation of stable cell lines. You can also CONTACT US with any question or enquiry you may have. We will be happy to discuss your needs in detail and design an appropriate plan of action.
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