Proteomics is the systematic identification and characterization of proteins, their structures and functions. Medicilon has extensive experience in the analyses of protein using advanced technology including mass spectrometry and bioinformatics. The facility provides support, equipment and expertise for analyzing complex protein mixtures. We work with each client to determine the best approach and services needed to fulfill a specific project goal, budget and timeline.
The word proteome comes from the combination of the words protein and genome, which means "a complete set of proteins expressed by a genome", that is, including the proteins expressed by a cell and even an organism. total protein. Proteomics essentially refers to the study of protein characteristics at a large-scale level, including protein expression levels, post-translational modifications, protein-protein interactions, etc., thereby obtaining information on disease occurrence, cell metabolism, etc. at the protein level. A holistic and comprehensive understanding of the process.
The study of proteome can not only provide a material basis for the laws of life activities, but also provide theoretical basis and solutions for the elucidation and overcoming of various disease mechanisms. Through the comparative analysis of the proteome between normal individuals and pathological individuals, we can find some "disease-specific protein molecules", which can become molecular targets for new drug design, or provide molecular markers for early diagnosis of diseases . Indeed, some of the best-selling drugs in the world are themselves proteins or target certain protein molecules. Therefore, proteomic research is not only a necessary work to explore the mysteries of life, but also brings huge benefits to the cause of human health. The study of proteomics is the characteristic of life science entering the post-gene era.
1) Identification of Proteins from Some Source
2) Measuring Relative Differences in Protein Expression
3) Finding Post-Translational Modifications of a Protein
Mass Spectrometry provides a variety of services related to protein fractionation, qualitative and quantitative mass spectrometry analysis, de novo protein sequencing and protein database searching. Protein sequencing and identification are routine procedures in the core. The core has a suite of state-of-the-art mass spectrometers and software available for detailed characterization of proteins and peptides, including post-translational modification analysis and accurate molecular weight determination.
Biomolecular Mass Spectrometry
– Liquid chromatography mass spectrometry to determine intact protein molecular weight, structure characterization and quantitation
– Electron Transfer Dissociation
– Matrix-assisted laser desorption/ionization with a time-of-flight mass spectrometer
Small Molecule Mass Spectrometry
– Liquid chromatography mass spectrometry with ion trap
– High resolution mas spectrometry using flow injection on LTQ Orbitrap with internal calibration
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