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Top 10 Global CDMO Enterprises| The Meaning of IND, NDA and ANDA| Top 10 Global Clinical Research Organizations in 2021
Feb 20,2021
Introduction to indirect immunofluorescence
What is immunofluorescence technology? Immunofluorescence technology is an immunoassay technique that combines the specificity of antigen-antibody reaction with the sensitivity and intuitiveness of fluorescent substance detection. What are the types of immunofluorescence technology? Fluorescent antibody staining technology: fluorescence microscopy technology, flow cytometry immunofluorescence technology (2) Fluorescence immunoassay: time-resolved immunoassay, fluorescence polarization immunoassay What are theRead more
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Introduction to indirect immunofluorescence
Feb 20,2021
Direct Fluorescent Antibody Test
Fluorescent Antibody Test is based on the principle of antigen-antibody reaction. Firstly, the known antigen or antibody is labeled with fluorescein to make fluorescent antibody, and then this fluorescent antibody (or antigen) is used as a probe to detect the corresponding antigen in the tissue or cell (Or antibody). The antigen-antibody complex formed in theRead more
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Direct Fluorescent Antibody Test
Feb 20,2021
Indirect immunofluorescence method for antibody detection
What is indirect immunofluorescence method for antibody detection? 1. Basic principles: The staining procedure is divided into two steps. In the first step, an unknown unlabeled antibody (specimen to be tested) is added to the known antigen sample, and the sample is incubated at 37°C for 30 min in a humidified box to fully bindRead more
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Indirect immunofluorescence method for antibody detection
Feb 20,2021
Immunofluorescence sample labeling detected by flow cytometry
Introduction: Commonly used fluoresceins for flow cytometry detection include FITC, TRITC, Cy3, Cy5, PE and PI. Among the above various fluorescent dyes, PE has the strongest fluorescence and is suitable for weakly expressing antigens; FITC is the cheapest and is suitable for strong Express antigen, wide application range. 1 Flow cytometer (Flow cytometer) is aRead more
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Immunofluorescence sample labeling detected by flow cytometry
Feb 19,2021
Indirect immunofluorescence detection of autoantibodies
The standard technique for autoantibody diagnosis is indirect immunofluorescence, which is characterized by strong specificity and obvious signal intensity contrast between positive and negative samples. Microscopic observation can accurately determine the distribution of fluorescence in tissues or cells. The position of the autoantigen determines the typical fluorescence pattern of its corresponding antibody, and the stainingRead more
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Indirect immunofluorescence detection of autoantibodies
Feb 19,2021
The latest progress and development trend of autoantibody indirect immunofluorescence detection technology
Autoantibody detection is an important method for diagnosing autoimmune disease (autoimmune disease, AID). Commonly used detection methods include indirect immunofluorescence (IIF), enzyme-linked immunoassay (ELISA), western blotting, etc., with the continuous innovation of biotechnology , Auto-antibody detection has been gradually applied to autoantibody detection by technologies such as magnetic bead particle chemiluminescence, immune microarray, and flowRead more
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The latest progress and development trend of autoantibody indirect immunofluorescence detection technology
Feb 19,2021
Precautions for the selection of immunofluorescence double-labeled antibodies
Double immunofluorescence When detecting two antigens at the same time in the same tissue or cell sample, double fluorescent staining is required. Double immunofluorescence labeling method (double immunofluorescence labeling method) is also divided into direct method and indirect method. Direct double immunofluorescence labeling Direct double immunofluorescence labeling: mix two different fluorescein-labeled antibodies (such as anti-ARead more
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Precautions for the selection of immunofluorescence double-labeled antibodies
Feb 19,2021
Fluorescein FITC-labeled antibody method
Immunofluorescence technology combines fluorescein such as fluorescein isothiocyanate (FITC) with the corresponding antibody (or antigen) in a chemical way, and combines the fluorescent-labeled antibody (or antigen) with the corresponding antigen in the specimen to form a fluorescent-labeled antibody -Antigen complex, observe with a fluorescence microscope. The presence of fluorescence under the microscope indicates the presenceRead more
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Fluorescein FITC-labeled antibody method
Feb 19,2021
Application of fluorescent antibody in clinical diagnosis and treatment
Autoimmune disease refers to a series of pathological immune responses produced by the body's immune effect or immune effector molecules against one's own tissues or cells, leading to a large class of diseases that damage their own tissues and organs. The pathogenesis is very complex and the clinical manifestations are variable. , Often involving theRead more
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Application of fluorescent antibody in clinical diagnosis and treatment
Feb 19,2021
Fluorescent Antibody Technique
Fluorescence immunoassay technology is one of the earliest developed labeling immunoassay techniques. For a long time, some scholars have tried to combine antibody molecules with some tracer substances, and use antigen-antibody reaction to locate antigenic substances in tissues or cells. Coons was equal to the first successful use of fluorescein for labeling in 1941. ThisRead more
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Fluorescent Antibody Technique