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Hot information：
Top 10 Global CDMO Enterprises| The Meaning of IND, NDA and ANDA| Top 10 Global Clinical Research Organizations in 2021
Apr 26,2021
Yeast two-hybrid technology and common problems
Yeast two-hybrid, proposed by Fields in 1989, is an experimental technique for studying the interactions of proteins in living cells under the eukaryotic model yeast. This system can not only detect the interactions between known proteins, but also discover unknown proteins that interact with known proteins. It is widely used in animal and plant proteinRead more
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Yeast two-hybrid technology and common problems
Apr 26,2021
What you need to know about the yeast two-hybrid system
With the rapid development of molecular biology research, especially the human genome project, many new techniques have emerged in order to adapt to the development trend of functional research on many genes or proteins. Yeast two-hybrid is one of them. Yeast two-hybrid is one of them. So let's briefly understand what is the yeast two-hybridRead more
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What you need to know about the yeast two-hybrid system
Apr 23,2021
Yeast Two-hybrid: Basic Principles of Experimental Technical Posts
The experiment of yeast two-hybrid mainly consists of three parts: basic principle, experimental operation and common problems.Let's start with the principles section. Yeast two-hybrid is one of the commonly used methods to detect protein interaction. The yeast two-hybrid system was first established by Fields and Song in studying the regulation of eukaryotic gene transcription. TypicalRead more
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Yeast Two-hybrid: Basic Principles of Experimental Technical Posts
Apr 23,2021
Encyclopedia of yeast two-hybrid experiment steps
The principle of yeast two-hybrid system The yeast transcriptional activator GAL4 is composed of two separable and functionally independent domains. There is a DNA binding domain (BD) at the N terminal, which consists of 147 amino acids.There is a transcription activation domain (AD) at C terminal, which consists of 113 amino acids.BD can be combinedRead more
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Encyclopedia of yeast two-hybrid experiment steps
Apr 20,2021
Those things about the toxicity of the drug hERG
Background        In recent years, many drugs have been withdrawn from the market due to sudden cardiac deaths. In the pre-clinical research phase of drugs, 24% of the drugs were discontinued due to cardiovascular side effects, and 45% of the drugs were withdrawn from the market due to cardiac side effects. The main reasons forRead more
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Apr 19,2021
Spero: FDA notified the suspension of its phase IIa clinical trial of SPR720 to treat NTM-PD
Spero Therapeutics (SPRO) has announced that the U.S. Food and Drug Administration (FDA) has notified the suspension of its SPR720 Phase IIA clinical trial. SPR720 is a new type of antibacterial agent that targets enzymes necessary for bacterial DNA replication. SPR720 comes from Vertex and is currently being developed by Spero as an oral therapyRead more
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Spero: FDA notified the suspension of its phase IIa clinical trial of SPR720 to treat NTM-PD
Apr 16,2021
Buffer replacement solution during protein purification
Buffer replacement during protein purification To ensure that the proteins are in the same buffer system as those used in the downstream purification process.That is, the pH value and salt concentration of the environment where the protein is located should be consistent with the purification process, in order to optimize the purification process. Increase theRead more
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Apr 15,2021
Protein purification method selection and principles
The purpose of protein purification is to separate the target protein from all the components of the cell lysate, while still retaining the biological activity and chemical integrity of the protein. The separation and purification of protein is an arduous and arduous task. It is necessary to select an appropriate purification method according to theRead more
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Protein purification method selection and principles
Apr 15,2021
How to choose the buffer in the protein purification process?
The choice of buffer in the process of protein preparation and purification is very important, and only a suitable buffer can ensure the activity and structural stability of the protein. When we purify the protein, the most important thing is to keep the protein from being inactivated during the purification process, or to minimize theRead more
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How to choose the buffer in the protein purification process?
Apr 14,2021
Precautions for protein extraction and purification
Precautions for protein extraction and purification When purifying any kind of protein, you must always pay attention to maintaining its stability and protecting its activity. There are some general precautions to keep in mind. They include: The operation should be carried out on ice or in a cold storage as far as possible.Don't be tooRead more
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Precautions for protein extraction and purification
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