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Hot information：
Top 10 Global CDMO Enterprises| The Meaning of IND, NDA and ANDA| Top 10 Global Clinical Research Organizations in 2021
Aug 04,2021
ELISA Experiment Guide: Indirect ELISA
Indirect ELISA Indirect ELISA is illustrated in the figure below and Figure 2. Phases I and II are similar to the direct system.Phase III involves adding unlabeled detection antibodies that are diluted in the buffer to prevent the proteins in the antiserum from nonspecifically attaching to the solid phase (the blocking buffer). It is thenRead more
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Aug 03,2021
ELISA Indirect Method
As we all know, enzyme-linked immunosorbent assay (ELISA) is a technique that uses antigen-antibody interaction to quantify proteins, hormones, antibodies or viral antigens. Up to now, more than 200 research papers related to ELISA and antibody detection have been published in journals around the world every year. Among them, there are more than 70 papersRead more
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ELISA Indirect Method
Aug 03,2021
Indirect ELISA procedure
Indirect ELISA procedure-Experimental materials 1. 96-well microplate 2. TMB Experimental reagents 1. Encapsulation solution (pH9.6) : Na2CO3 0.17g, NaHCO3 0.28g, add distilled water to 100ml.4 ℃. 2. Washing solution (PBST): KH2PO4 0.2g, KCl 0.2g, NaCl 8.0g, Na2HPO4·12H2O 2.9g, Tween-20 0.5mL, add deionized water to 1000mL, adjust pH to 7.2-7.4. 3. Substrate reaction liquid: (1) SubstrateRead more
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Indirect ELISA procedure
Aug 02,2021
Drug cardiotoxicity research technique
Cardiotoxicity caused by some drugs during use is one of the life-threatening toxic side effects of patients. For example, adverse cardiac events caused by antiarrhythmic drugs have been reported from time to time. Although the incidence of such events is low, the risk is high, and the main manifestations are To prolong the QT intervalRead more
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Drug cardiotoxicity research technique
Aug 02,2021
Drug Safety Evaluation
Medicilon has a professional team and practical experience in drug safety evaluation, and can provide high-quality data and fast turnaround to support various drug safety evaluation studies. Toxicology studies can be performed in accordance with non-GLP or GLP standards. Our research platform has been rated as the Shanghai R&D public service platform. Medicilon drug safetyRead more
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Drug Safety Evaluation
Aug 02,2021
Why should GLP be implemented in new drug research?
GLP, Good Laboratory Practice, is a standard for safety evaluation experiments conducted on medicines, pesticides, food additives, cosmetics, etc., involving all aspects of laboratory work. For drug development, the implementation of GLP in pharmacology and toxicology research can guarantee the reliability, completeness and repeatability of experimental results. The top institutions in the ranking of ChineseRead more
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Why should GLP be implemented in new drug research?
Jul 31,2021
Bioanalytical Research Method
With the rapid development of the pharmaceutical industry, the research of biotechnology drugs has also entered a higher level of platform. Biotechnology drugs refer to therapeutic drugs produced through recombinant DNA technology or other innovative biotechnology-based biotechnology, which mainly include cytokines, plasminogen activators, recombinant plasma factors, growth factors, antibodies, vaccines and oligonucleotide drugs It isRead more
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Bioanalytical Research Method
Jul 31,2021
Biological analysis challenges of chemical drugs
New drug research and development is accompanied by high risks, high investment, and low returns. During this period, two important issues must be repeatedly verified: Is it safe and effective? Pharmaceutical R&D workers need to conduct systematic research on drugs through various preclinical and clinical trials to reveal or confirm the effects of test drugs,Read more
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Biological analysis challenges of chemical drugs
Jul 31,2021
Discovery of hit compounds
The discovery of hit compounds determines the starting point of the entire drug discovery process and is of vital importance. The confirmation stage of the hit compound is as follows: Exclude compounds with potential reactivity, interference with testing, or self-aggregation. Repeated activity test: For the selected target, the active compound obtained by high-throughput screening isRead more
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Discovery of hit compounds
Jul 30,2021
Pre-formulation Research of Generic Drugs
The purpose and importance of pre-prescription research The purpose of the Pre-formulation research is to provide the basis for the prescription process design, and to provide a clear mechanism of targeted solutions to the problems in the prescription process research. The quality characteristics of the final product are largely determined by the nature of theRead more
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Pre-formulation Research of Generic Drugs
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