Fluorescent In Situ Hybridization (Fish) Services

Medicilon offers a full line of Fluorescence in situ Hybridization (FISH) services, from standardized testing of validated assays to custom development of new assays. Drawing on many years of experience and in-depth knowledge, we guarantee that you will get the best FISH services from us for the ultimate success of your scientific research.

FISH, also known as whole chromosome painting, is valuable in defining rearrangements among different chromosomes and the origin of ring and marker chromosomes that are not clearly deciphered by karyotype analysis.

Data For Fish Services:

Detailed descriptions of methods, results and references.
Publication-ready photographs with figure legends.
Publication-ready diagrams (for gene mapping).
The following picture examples are provided.

Deliverable:

Raw data together with analyzed data in MS Excel format.
Images in JPEG format.
Project update and summary in MS PowerPoint format.
Final report in MS Word format (including  Purpose, Methods, Results and Data analysis).

What Is FISH?

Fluorescence in situ hybridization(FISH) service is a molecular technique that is often used to identify and enumerate specific microbial groups. This technique can be used to determine, with the presence or absence of a fluorescent signal, whether specific genetic elements exist in a sample. This can be useful for determining if microbes have a particular gene present and/or if that gene is being expressed under a given set of conditions. Fluorescent probes are designed to attach to specific genetic regions of microbes that will differentiate them from other groups. When these probes are applied a fluorescent microscope can be used to detect the presence or absence of individual microbial groups. Another sister technique, called Flow-Cytometric Analysis (FCM), can also be carried out when fluorescent tags are applied to microbial populations. Fluorescent signal is used to count or sort individual genotypes out of groups of cells.

When to use:

To confirm findings and refine breakpoints detected by g-banded karyotyping.
To confirm findings and localize genomic gains detected by aCGH analysis.
As a screen for microdeletions/duplications of known targets.
As a screen for aneuploidies other than X, 8, 12, and 17.

Applications of In Situ Hybridization

Microbiology (classic target – 16S rRNA) – morphology and population structure of microorganisms
Pathology (pathogen profiling, abnormal gene expression)
Developmental Biology (gene expression profiling in embryonic tissues)
Karyotyping and Phylogenetic Analysis (unique FISH patterns on individual chromosomes, chromosomal aberrations)

Physical Mapping (mapping clones on chromosomes and direct assignment of mapped clones to chromosomal regions associated with heterochromatin or euchromatin)

Contact us

Email : marketing@medicilon.com.cn
Tel : +86 021 58591500

Tips : Above is part of fluorescent in situ hybridization service. You can also CONTACT US with any question or enquiry you may have. We will be happy to discuss your needs in detail and design an appropriate plan of action.

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