Flow Cytometry Test Services

What is Flow Cytometry?

• Flow – refers to a fluid stream

• Cyto – refers to a cell

• metry – refers to measurement.

The Flow Cytometry department performs a wide variety of testing which includes immunophenotyping of cells used for the diagnosis of leukemia/lymphoma, CD4 counts for persons living with HIV/AIDS, platelet antibodies and platelet reticulocytes.

Flow Cytometry Services

Flow Cytometry Services including:

• Immunophenotyping

• Cell Viability

• Cell Cycle Analysis

• Apoptosis

  1. Annexin V

  2. Mitochondrial Membrane Potential

  3. Cytochrome C

  4. Intracellular Calcium Change

  5. Caspase Activity

  6. DNA Fragmentation

• Cell Tracking and Proliferation

• Fluorescent Protein Measurement

• Intracellular Measurements: Cytokines, Transcription Factors, Phosphoproteins

• Purification of any fluorescently tagged cells in a complex mixture based on target specificity for further research

• Cloning and Purification of Cells on Multi-Well Plates, Hybridoma Sorting

• Isolating Cells for Functional Analysis, Protein Analysis

• Purify Cells for Transplantation into Laboratory Animals

• General Enrichment of Cells

• Sorting for PCR Analysis

Protocols Are Available For:

• Direct staining of cells applicable where the fluorophore is directly linked to the primary antibody

• Indirect staining of cells applicable when using unconjugated or biotin-conjugated monoclonal and polyclonal antibodies

• Intracellular staining methods for intracellular antigens and cytokines

• DNA Staining for Cell Cycle Analysis

• Cell Preparation

Medicilon scientists have wealth of knowledge and experience in using flow cytometry to tackle important assay development and research challenges in both genetic toxicology and high-throughput screening.

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