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Insect Baculovirus Expression Vector System, referred to as IBEVS (Insect Baculovirus Expression Vector System), is one of the four major genetic engineering expression systems. Since it does not have any animal virus fragments that are potentially pathogenic to mammals, the insect baculovirus expression system has been highly valued since its advent.
The U.S. Food and Drug Administration’s evaluation of BEVS is “The FDA recognizes the products produced by BEVS, which provides a prospect for the emerging biopharmaceutical industry, especially for the development of human disease treatments and vaccines, improving human health and improving the lives of millions of people. The quality is even more so”.
Compared with E. coli, yeast, and mammalian cell expression systems that will be introduced later, IBEVS has special research value in the following four aspects:
1
As a super efficient eukaryotic gene expression vector, it can produce useful engineering protein
2
As a genetically engineered virus insecticide, improve the efficiency of pest control
3
Study the structure and function of the baculovirus genome
4
Study the regulation mechanism of eukaryotic gene expression
In recent years, the insect baculovirus expression vector system has become a powerful and popular tool for the production and research of various prokaryotic and eukaryotic proteins. As a protein expression service expert around you, Medicilon will tell you all the advantages of the most classic Bac-to-Bac baculovirus expression system:
Since the recombinant virus is separated by blue and white spot screening, there is no problem of wild-type and non-recombinant virus contamination, so traditional and cumbersome plaque analysis is not required to purify recombinant virus.
The time required for the construction of the recombinant virus is greatly shortened and the recombination efficiency reaches 100%.
The foreign gene can be folded correctly in insect cells, and the expressed target protein is closer to the natural protein in structure, and has complete biological functions.
Insect cells can process and modify post-translational proteins such as phosphorylation, glycosylation, acylation, signal peptide excision, and peptide cleavage and decomposition.
Baculovirus can accommodate inserts of large molecules and can package large gene fragments.
The baculovirus expression system has the ability to express multiple genes simultaneously in the same cell. Different recombinant viruses can be used to infect cells at the same time, or two foreign genes can be cloned simultaneously on the same transfer vector. The expression product can be processed. The formation of active heterodimers or multimers.
Insect cells grow in suspension, which is easy to scale up and cultivate, which is conducive to large-scale expression of recombinant protein.
Baculovirus is non-infectious to vertebrates. Existing studies also show that its promoter is not active in animal cells, so it is significantly better than other systems when expressing oncogenes or potentially toxic proteins.
Baculovirus Expression System (Also Called Insect Cell Expression System)