Anthraquinone compounds are one of the important active components of aloe, which mainly include aloe emodin, chrysophanol, emodin, rhein acid and emodin methyl ether. A large number of cell experiments have shown that the anthraquinone compound aloe emodin can inhibit tumor cell proliferation and induce tumor cell apoptosis by blocking cell cycle. Therefore, the research and development of aloe emodin antitumor drugs has attracted the attention of medical researchers.
Some researchers have studied the effects of aloe-emodin on endometrial cancer cells through cell experiments. Others have explored the effects and mechanisms of aloe-emodin on telomerase activity in human liver cancer HepG2 cells. The researchers used telomere repeat sequences. Amplification technology (TRAP) was used to detect the telomerase activity of HepG2 cells and found that aloe-emodin can inhibit the telomerase activity of HepG2 cells by down-regulating the expression of cmyc and hTERT. For the development of new drugs, enzyme activity testing services can improve the efficiency of scientific research practice and the quality of result innovation. Medicilon provides enzyme activity testing services. Its biological department has rich and extensive experience in the field of in vitro biology, through enzyme level determination, cell level determination, cell biology, biochemistry, in vitro isotope determination, stable cell line establishment, gene knockout In addition, RNAi and MicroRNA technologies, etc., provide a complete set of biological services.
Researchers explored the effect of aloe-emodin on the migration and invasion of human endometrial cancer cells HEC-1-B [1]. The method is that HEC-1-B cells are cultured and divided into control group and experimental group. The control group cells are not treated with any treatment, and the experimental group is treated with aloe-emodin (30μmol/L) for 24h. The cell scratch test was used to detect the effect of aloe-emodin on the migration of HEC-1-B cells; the Transwell chamber experiment was used to detect the effect of aloe-emodin on the invasion of HEC-1-B cells. Results Compared with the control group, the width of the scratches in the experimental group after 24 hours was significantly greater than that of the control group. The number of permeable cells in the experimental group was significantly reduced after 24 hours. Therefore, aloe-emodin can inhibit the migration and invasion of human endometrial cancer cells. The cell scratch test is a routine cell test service. As a simple and easy method to detect cell movement, the test cost is low, and it can be used to detect the invasion and metastasis ability of adherent tumor cells.
Researchers have also conducted research on the effects of aloe-emodin (AE) on the proliferation and apoptosis of endometrial cancer (EC) cells through the Notch signaling pathway and its prognostic significance [2]. Researchers purchase endometrial cancer cell line RL-952, use cell proliferation and apoptosis experiments to study the effect of AE on RL-952; use cell migration and invasion experiments to study the effect of AE on the prognosis of EC patients; use WB, qRT -PCR to study the effect of AE on the expression of proteins related to Notch signaling pathway in tumor cells. The results showed that AE inhibited cell proliferation, migration and invasion, and induced apoptosis; AE increased the expression of Notch1, Notch3 mRNA and protein, inhibited the expression of Jagged1, DLL4 mRNA and protein, and activated the Notch signaling pathway. Therefore, AE inhibits the proliferation of EC tumor cells, induces apoptosis, and inhibits cell migration and invasion by activating the Notch signaling pathway. The above results provide relevant theoretical basis for improving the treatment of EC patients.
It has been found that immortal cells and tumor cells can survive for a long time, and telomerase plays an important role. Telomerase has a very close relationship with cell proliferation, differentiation and immortality, and has become one of the current hot spots in basic research on tumors and aging. Some researchers have observed the effect of aloe-emodin (AE) on the telomerase activity of human liver cancer HepG2 cells, and explored its possible molecular mechanism [3]. Methods HepG2 cells in logarithmic growth phase were treated with AE at the final concentration of 0, 10, 30, and 50 μmol/L for 24 hours; HepG2 cells were collected, and the telomerase of HepG2 cells was determined by the telomere repeat amplification technique (TRAP) Activity, Western blotting and PCR were used to detect hTERT, c-myc protein and gene expression levels in HepG2 cells.
Results HepG2 cells were treated with AE at final concentrations of 0, 10, 30, and 50 μmol/L for 24 hours, and the telomerase activities of HepG2 cells were 0.707±0.001, 0.546±0.008, 0.338±0.001, 0.214±0.009, respectively; as the AE concentration increased , The telomerase activity of HepG2 cells decreased, and the difference in telomerase activity of HepG2 cells at 30, 50μmol/L and 0μmol/LAE was statistically significant (all P<0.05). With the increase of AE concentration, the expression levels of hTERT, c-mycmRNA and protein in HepG2 cells decreased; among them, hTERT, c-mycmRNA and protein were at 50μmol/L, hTERTmRNA was at 30μmol/L, and the difference was statistically significant from 0μmol/L (P<0.05). Therefore, AE can inhibit the telomerase activity of HepG2 cells by down-regulating the expression of cmyc and hTERT.
In addition to the above studies, researchers have also found that aloe-emodin can induce apoptosis in lung cancer cell line CH27. Using different concentrations of AE to act on tumor cells will find a dose-time-dependent inhibition relationship, and at the same time find this inhibition The effect is most significant when the concentration of AE is 50um. In short, there are still many researches on the effects of aloe-emodin on tumor cells. With the in-depth study of its anti-tumor activity, its various anti-cancer mechanisms will gradually be elucidated, which will help the research and development of aloe-emodin anti-cancer drugs.
[1] The effect of aloe-emodin on the migration and invasion of human endometrial cancer cells HEC-1-B [J].
[2] Aloe-emodin activates the Notch signaling pathway to affect the proliferation and apoptosis of endometrial cancer cells and its prognostic significance [J].
[3] The effect of aloe-emodin on the telomerase activity of human liver cancer HepG2 cells and its mechanism [J].
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