Protein Folding Findings May Lead to Better Therapies for a Range of Human Diseases

    Though research on protein folding has progressed over the past few decades toward better understanding of human metabolism and the diseases associated with misfolding, important discoveries are still being made by teams who can bring special techniques and tools to bear on these complex cellular processes.

    Scientists at the University of Massachusetts Amherst report for the first time how a key protein in the blood coagulation pathway folds to a higher-energy (cocked) state. As a result, the protein can function as a sort of molecular mousetrap and generate the work required to perform physiologically important functions.

    The study (“Cellular folding pathway of a metastable serpin”), which appears in PNAS, advances understanding of genetic forms of thrombosis, emphysema, cirrhosis of the liver, neurodegenerative diseases, and inflammation, among others, according to the researchers.

    “Generally these proteins don’t have a lot of mobility, but this particular protein is folded like a spring-loaded trap, so it is stable at a higher energy state,” explains Daniel Hebert, Ph.D., professor of biochemistry and molecular biology. “When it’s needed, it fires from a loaded position and undergoes the big structural changes needed to carry out its inhibitory function against target proteases. We can explain now for the first time how it folds in live cells into an active state and how it avoids folding to a more stable but inactive state.”

    He and colleagues used radio-labeled, pulse-chase techniques to follow the serpin antithrombin III (ATIII) through time as it folded. They also used antibodies to isolate it and gel electrophoresis to analyze its expression, folding, and function. Dr. Hebert notes that it takes about 1 hour overall for cells to synthesize, fold, and secrete the serpin ATIII, but he and his team found that in the critical first few minutes of this process a key positioning of the mousetrap’s trigger wire takes place; the rest of the trap builds around it, allowing it to reach the loaded higher-energy conformation.

    “We found it a bit surprising that the part of the protein that is synthesized last is locked into place early in the folding process, helping to guide later structure formation. But on reflection it makes sense, because the amount of energy it would take to cock the trigger at the end would be prohibitive. So the protein starts out cocked, and goes on from there,” he points out.

    The research team introduced a number of disease-associated mutations and watched where proteins containing these mutations diverged from normal folding. Their research not only introduces and defines for the first time the early stages of the normal pathway, but it also describes how 13 mutations found in humans affect ATIII folding. They are already working with medical geneticists on identifying pharmacological chaperones to help mutated, misfolded proteins fold correctly and develop better constructs for gene therapies, says Dr. Hebert.

    “We figured out the key for this protein to fold correctly,” he continues. “We utilized a type of serpin with a particular kind of covalent bond that would give us an experimental advantage that we could exploit to map the folding reaction. We chose this target deliberately and it allowed us to sort out the earliest stages that provided the key to how the spring gets loaded.”

    The serpin family is an important class of proteins that control diverse biological processes, and, unlike other proteins, they don’t always fold to their most stable state, notes Dr. Hebert.  In fact, ATIII folds to a higher-energy state, which is harder to get to and offers more chances for problems to arise during protein structure formation. Misfolded and partly folded serpins will never make it out of the cell, leading to lower protein levels in blood or to the buildup of a toxic conformation, either of which is associated with disease.

    The authors say that their data identifies steps essential to ATIII folding, and the findings serve as a jumping off point to explore both in vitro and in vivo folding of related serpins. Results highlight possible answers to questions about how serpins successfully navigate their folding landscape to produce adequate amounts of functional metastable protein and avoid deleterious aggregation, and suggest promising areas for future work.