Flow Sorting

Cell sorting

• Cell sorting is a technology that separates a specific cell subgroup from a mixed cell sample according to the characteristics of the cells, and is an important topic in cytology research.
  When it is necessary to perform functional analysis on a specific cell, researches such as cell molecules analyzed by ELISA, cell co-culture and detection of cell function are based on the premise of obtaining high-purity target cells.

Flow Cytometry

• Flow cytometric sorting is based on the detection of cell phenotypes by flow cytometry, using charge sorting technology to achieve the separation of a variety of specific phenotype cells.  It has the advantages of multiple sorting parameters, multiple groups, high purity, and strong flexibility.  After sorting, cells can be used for further biological research such as single-cell sequencing.

Instruments and Equipment

• SONY Flow Cytometer SH800

  It has fully automatic adjustment of the optical axis of the laser beam and electronic timing functions for cell sorting, eliminating the need for complex settings and adjustments.  It is convenient to set up, which can significantly improve the work efficiency of researchers.                                       Using the newly developed plastic cell sorting chip, chips with different nozzle diameters are easy to be replaced and installed.  The chips can be selected according to the type and size of the cells to be tested to meet the needs of different projects.                                        Equipped with software with a user interface, it is easy to operate, and can objectively and quickly analyze the information of each cell.                 

  

Sorting Pattern Diagram

Medicilon Case Study

• 
  NK Cell GFP Positive Sorting
  
  K562 GFP Positive Sorting

Related Platform:

• 
  Flow Cytometry Technology Platform

FAQs

• What is Flow Cytometry?

  Flow Cytometry (FCM) is a technology that allows for rapid, accurate, objective, and simultaneous analysis and quantification of multiple physical and biological characteristics of individual cells in rapid linear flow conditions. It also enables sorting of specific cell populations.

  A flow cytometer is a new high-tech instrument integrating laser technology, electronic physics, photodetection technology, electronic computer technology, cell fluorescence chemical technology and monoclonal antibody technology.

• What is Flow Cytometer?

  Flow Cytometer is a new high-tech instrument integrating laser technology, electronic physics technology, photoelectric measurement technology, electronic computer technology, cell fluorescence chemical technology and monoclonal antibody technology.

• Advantages of Flow Cytometry

  • Fast detection speed and ability to analyze large sample volumes

  Flow cytometry distinguishes itself by analyzing a large number of cells in a very short period of time. It can measure tens of thousands of cells per second.

  • Simultaneous analysis of multiple features of individual cells

  By using different fluorescein-labeled monoclonal antibodies or fluorescent dyes, flow cytometry can provide multiple pieces of information about single cells.

  • Capability to detect diverse types of samples

  Flow cytometry can analyze various types of samples including different types of cells (such as peripheral blood, bone marrow, fine needle aspirates, lavage fluids, solid tissues, cells from suspension or adherent cultures), microorganisms, and synthetic microspheres.

• 所有研究均在同一园区内实验室有序完成，切实保证实验进度和项目沟通的高效性，占据数据获取和评估的行业优越性。