Protein binding: plasma, brain tissue, microsomal protein, FBS; Erythrocyte /plasma partition ratio
Optimization permeability and transport: Caco - 2. MDCK - MDR1 / BCRP, OATs/OCTs/OATPs
In vitro metabolic DDI: P450 inhibited TDI, P450 induced /PXR, metabolic enzyme phenotype.
Metabolic stability: microsomes, S9, hepatocytes, plasma, whole blood
Metabolite Screening and Identification: in vitro, in vivo, and GSH Trapping
In vivo PK: rat, mouse, dog, monkey, pig, rabbit box/single administration
Routes of administration: IV/IJVC/SC/TD/IM/IP/PO/SL/IN/IVT
Continuous cross/single point blood sampling
BBB(homogenate /CSF), Tissue distribution excretion (BDC), in vivo DDI (ABT)